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为观察神经标志物PGP 9.5和S-100蛋白在先天性巨结肠(HD)中的表达,采用一抗为抗PGP 9.5和抗S-100蛋白的PAP免疫组织化学方法,探讨其在临床诊断中的意义。结果:①在对照组结肠壁神经丛中可见染色深浅不一的PGP 9.5免疫反应阳性神经节细胞,神经纤维均匀分布在肠壁各层;神经节细胞胞体不表达S-100蛋白,表现为细胞状“空白区”。②HD结肠壁神经发育异常,PGP 9.5和S-100蛋白免疫反应性神经纤维明显增生,分布紊乱,未见有PGP 9.5阳性神经节细胞;在神经丛中,增生的S-100蛋白阳性神经纤维中偶见有细胞状的“空白”区。提示结肠壁神经发育异常是HD的主要病理生理变化,神经丛中PGP 9.5阳性反应的细胞团块和S-100蛋白染色的神经丛中细胞状“空白”区,可特征性地提示神经节细胞的存在,用于HD的临床诊断敏感度高。
To observe the expression of neuronal markers PGP 9.5 and S-100 in Hirschsprung’s disease, PAP immunohistochemistry with primary antibodies against PGP 9.5 and anti-S-100 was used to investigate its clinical significance Meaning. Results: ① In the control group, PGP 9.5 immunoreactive ganglion cells were observed in the plexus of the colon wall, and the nerve fibers were evenly distributed in the layers of the intestinal wall. The ganglion cell bodies did not express S-100 protein, Shaped “blank area.” (2) dysplasia of HD colon wall, PGP 9.5 and S-100 protein immunoreactive nerve fibers were significantly proliferated and disordered, and no PGP 9.5-positive ganglion cells were seen; in the nerve plexus, hyperplastic S-100 protein positive nerve fibers Occasionally cell-like “blank” area. Suggesting that the abnormal development of the colonic wall is the major pathophysiological change of HD. The cell-like “blank” region in PGP 9.5-positive cell mass in the nerve plexus and in the plexus stained by S-100 protein may characteristicly suggest that ganglion cells The presence of high sensitivity for the clinical diagnosis of HD.