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本文报告从150g人心肌中得到电泳纯和免疫纯的肌酸激酶MB和MM同工酶(OK-MB和CK-MM)5.1mg和61mg,比活性分别是1232u/mg蛋白和408u/mg蛋白。同时,还研究了CK-MB和CK-MM的部分理化性质和免疫学性质。此外,制备成功CK-MB和CK-MM抗血清。经联结标记法标记~(125)I,比放射性CK-MB约11.1×10~6Bq/μg,CK-MM约5.92×10~6Bq/μg,50％结合率的CK-MB抗血清稀释度1:75000,CK-MM1:15000,亲和常数(K值)CK-MB2.7×10~(11)L/mol,CK-MM3.1×10~9L/mol,均具有理想的抗体特异性。适用于CK-MB和CK-MM的放射免疫分析(RIA)。
Herein, 5.1 mg and 61 mg of creatine kinase MB and MM isoenzymes (OK-MB and CK-MM) and electrophoresed pure and immunologically pure creatinine MBK were obtained from 150 g human myocardium with specific activities of 1232 u / mg protein and 408 u / mg protein . At the same time, some physical and chemical properties and immunological properties of CK-MB and CK-MM were also studied. In addition, successful CK-MB and CK-MM antisera were prepared. The concentration of CK-MB antiserum, labeled with 125I I, was about 11.1 × 10 ~ 6Bq / μg for radioactive CK-MB and about 5.92 × 10 ~ 6Bq / μg for CK-MM. : 75000, CK-MM1: 15000, K-MB2.7 × 10 ~ (11) L / mol and CK-MM3.1 × 10 ~ 9L / mol for the affinity constant (K value) . Radioimmunoassay (RIA) for CK-MB and CK-MM.