论文部分内容阅读
目的:研究人反义Tankyrase逆转录病毒载体的构建,探讨以tankyrase为靶点的肿瘤基因治疗的可能性。方法:将Tankyrase的cDNA反向插入逆转录病毒载体pLXRN中,转染包装细胞PT67后获得反义重组病毒。结果:经HindⅢ酶切鉴定,反义重组子可产生200 bp的条带,与理论计算值完全一致。结论:成功构建了人Tankyrase的反义逆转录病毒载体。
Objective: To study the construction of antisense Tankyrase retroviral vector and to explore the possibility of tumor gene therapy targeting tankyrase. Methods: The cDNA of Tankyrase was reversely inserted into retrovirus vector pLXRN, and antisense recombinant virus was obtained after transfection of packaging cell PT67. Results: After digestion with Hind Ⅲ, the antisense recombinant protein produced a 200 bp band, which was completely consistent with the calculated value. Conclusion: Human Tankyrase antisense retroviral vector was successfully constructed.