【摘 要】
：
Skeletal muscle differentiation is a highly coordinated process that involves many cellular signaling pathways and microRNAs(miRNAs).A group of muscle-specific miRNAs has been reported to promote myogenesis by suppressing key signaling pathways for cell g
【机 构】
：
MOE Key Laboratory of Gene Function and Regulation,State Key Laboratory of Biocontrol,School of Life
论文部分内容阅读
Skeletal muscle differentiation is a highly coordinated process that involves many cellular signaling pathways and microRNAs(miRNAs).A group of muscle-specific miRNAs has been reported to promote myogenesis by suppressing key signaling pathways for cell growth.However,the functional role and regulatory mechanism of most non-muscle-specific miRNAs with stage-specific changes during differentiation are largely unclear.Here,we describe the functional characterization of miR-101 a/b,a pair of non-muscle-specific miRNAs that show the largest change among a group of transiently upregulated miRNAs during myogenesis in C2C12 cells.The overexpression of miR-101a/b inhibits myoblast differentiation by suppressing the p38/MAPK,Interferon Gamma,and Wnt pathways and enhancing the C/EBP pathway.Mef2a,a key protein in the p38/MAPK pathway,was identified as a direct target of miR-101a/b.Interestingly,we found that the long non-coding RNA(lncRNA)Malat1,which promotes muscle differentiation,interacts with miR-101a/b,and this interaction competes with Mef2a mRNA to relieve the inhibition of the p38/MAPK pathway during myogenesis.These results uncovered a“braking”role in differentiation of tran-siently upregulated miRNAs and provided new insights into the competing endogenous RNA(ceRNA)regulatory mechanism in myoblast differentiation and myogenesis.
其他文献
Isolation of E-1,2-bis(4-bromophenyl)-1,2-diphenyl-ethylene from the E/Z isomer mixture obtained by a McMurry coupling reaction and reaction of this isomer with imidazole followed by N-alkylation with nBuBr and anion exchange yielded the bisimidazolium te
Flavin-dependent halogenases(FDHs)are well known to introduce carbon halide bonds(mainly C-Cl and C-Br)into natural products with the assistance of a partner protein flavin reductase to generate reduced flavin(FADH2 or FMNH2).Compared with the common chlo
Atomic sites co-catalyst(ASC)on photocatalytic materials possesses an attractive prospect to promote charge carrier separation and tune surface reaction kinetics,yet the synthesis of earth-abundant ASC under low temperature remains a great challenge.Here,
Open metal site(OMS)seated in a luminescent lanthanide(Ln)metal center offers an opportunity for rationally tuning the spectroscopic behavior of lanthanide-organic frameworks aiming for a wide range of sensing applications.However,given the spherical natu
Tiacumicin B(also known as fidaxomicin or difimicin)is a marketed 18-membered macrolide antibiotic for the treatment of Clostridium difficile infections.Tiacumicin B is structurally characterized with two chlorine atoms substituted on the aromatic ring,wh
Catalytic generation of cobalt enolates through ring-opening of easily accessible cyclopropanols,subsequent β-hydride elim-ination and site-selective reinsertion followed by diastereo-and enantioselective additions to aldimines is presented.Such a process
Nitrogen-containing motifs are widely present in natural products,bioactive molecules,and drugs.Accordingly,effective synthetic methods with high efficiency and diversity are highly desirable.Here,we present the invention of a facile,visible light-mediate
The developments of hydrogen sources stand at the forefront of asymmetric reduction.In contrast to the well-studied alcohols as hydrogen sources via β-hydride elimination,the direct utilization of the proton of alcohols as a hydrogen source for activator-
Achieving real-time,continuous and long-term monitoring of dopamine(DA)in vivo is essential for revealing brain functions and preventing and treating neurogenic diseases.However,it remains challenging to achieve a low limit of detection(LOD)and high neuro
Neutrophils are crucial for immunity and play important roles in inflammatory diseases;however,mouse models selectively deficient in neutrophils are limited,and neutrophil-specific diphtheria toxin(DT)-based depletion system has not yet been established.I