论文部分内容阅读
为探明我国大麦亲本材料的遗传背景和群体结构特点,提高大麦种质材料的利用效率,选用50对多态性好的SSR引物对63份大麦亲本材料进行遗传多样性和群体结构分析。结果表明,50对引物共检测到119个等位变异,平均每个位点的等位变异数为2.38个,变异范围为2~5;平均有效等位变异数为1.75,有效等位变异所占比重为74.16%;Shannon’s信息指数和多态信息含量(PIC)的变幅分别为0.082~1.383和0.031~0.701,平均为0.59和0.308。遗传相似系数(GS)变异范围为0.652~0.990,聚类分析表明63个大麦亲本材料在GS值为0.694水平上聚为3个大类,基于数学模型的群体结构可分为4个亚群。本研究涉及的大部分材料亲缘关系较近,需要引入新种质来拓展亲本的遗传基础。
To identify the genetic background and population structure characteristics of barley parents in China and improve the utilization efficiency of barley germplasm materials, 50 SSR primers with good polymorphism were used to analyze the genetic diversity and population structure of 63 barley parents. The results showed that a total of 119 alleles were detected with 50 pairs of primers. The average number of alleles per locus was 2.38 and the range of variation was 2 ~ 5. The average number of effective alleles was 1.75, and effective alleles Accounting for 74.16% of the total. Shannon’s information index and polymorphism information content (PIC) ranged from 0.082 to 1.383 and from 0.031 to 0.701, with an average of 0.59 and 0.308, respectively. The genetic similarity coefficient (GS) ranged from 0.652 to 0.990. Cluster analysis showed that the 63 barley parent materials clustered into three groups at a GS value of 0.694. The population structure based on the mathematical model can be divided into four subgroups. Most of the materials involved in this study have a close genetic relationship and need to introduce new germplasm to expand the genetic basis of their parents.