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This experiment sought to observe the migration and distribution of bone mesenchymal stem cells transfected with the cytosine deaminase gene (BMSCs-CD/eGFP) after transplantation in vivo through three pathways. In addition, we examined the tropism of these cells to glioma. Intracranial C6 glioma models were established in Sprague-Dawley rats using an intracranial stereotactic inoculation method. When tumors were 7 days old, rats were inoculated with 1×106 BMSCs-CD/eGFP cells via the tumor-bearing internal carotid artery, the contralateral hemisphere and the tumor-bearing glioma. Fluorescence microscopy revealed that BMSCs-CD/eGFP exhibited a strong capacity for migration to tumors. BMSCs-CD/eGFP transplanted via the tumor-bearing internal carotid artery were observed to distribute in glioma tissues. BMSCs-CD/eGFP inoculated via the ipsilateral glioma mainly located within and at the edge of glioma tissues. BMSCs-CD/eGFP inoculated via the contralateral hemisphere mainly distributed at the proximal end of the tumor at the incubation site.
This experiment sought to observe the migration and distribution of bone mesenchymal stem cells transfected with the cytosine deaminase gene (BMSCs-CD / eGFP) after transplantation in vivo through three pathways. In addition, we examined the tropism of these cells to glioma. Intracranial C6 glioma models were established in Sprague-Dawley rats using an intracranial stereotactic inoculation method. When tumors were 7 days old, rats were inoculated with 1 × 10 6 BMSCs-CD / eGFP cells via the tumor-bearing internal carotid artery, the contralateral hemisphere and the tumor-bearing glioma. Fluorescence microscopy revealed that BMSCs-CD / eGFP showed a strong capacity for migration to tumors. BMSCs-CD / eGFP transplanted via the tumor-bearing internal carotid artery were observed to distribute in glioma tissues. inoculated via the ipsilateral glioma mainly located within and at the edge of glioma tissues. BMSCs-CD / eGFP inoculated via the contralateral hemisphere mainly distributed at the proximal end of the tumor at the incubation site.