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目的 建立同时测定血清中复方氯唑沙宗片两组分浓度的HPLC。方法 血清样品经饱和硫酸锌溶液沉淀 ,乙醚提取 ,氮气挥干 ,加甲醇溶解进样 ,以HypersilODSC1 8色谱柱 ,甲醇 水 (4 5∶5 5 )为流动相 ,非那西丁为内标 ,在波长 2 80nm处检测。结果 氯唑沙宗在 1～ 32 μg·ml- 1 、对乙酰氨基酚在 1.2～ 38.4μg·ml- 1 浓度范围内 ,样品峰面积比与浓度呈良好线性关系 ,r氯 =0 .9994,r对 =0 .9999。最低检测浓度分别为 0 .5和 0 .6 μg·ml- 1 ,平均回收率分别为 95 .3 %和 91.6 %。RSD分别为 6 .0 % ,5 .1%。结论 此法可同时测定复方氯唑沙宗片两组分的浓度 ,为药物动力学和生物利用度研究提供了简单可行的方法
Objective To establish a HPLC method for the simultaneous determination of two components in compound chlorzoxazone tablets in serum. Methods Serum samples were precipitated with saturated zinc sulfate solution, extracted with diethyl ether, evaporated to dryness under nitrogen and dissolved in methanol. The mobile phase was Hypersil ODSC 18 and methanol water (4 5:5 5) Detection at a wavelength of 280nm. Results Chlorzoxazone showed a good linear relationship with the concentration of 1 ~ 32 μg · ml-1 and acetaminophen at a concentration of 1.2 ~ 38.4 μg · ml-1, with a linear relationship of r = 0.9994, r = 0 .9999. The minimum detectable concentrations were 0.5 and 0.6 μg · ml -1, respectively. The average recoveries were 95.3% and 91.6%, respectively. RSD were 6 .0%, 5 .1%. Conclusions This method can be used to determine the concentration of two components in compound chlorzoxazone tablets at the same time. It provides a simple and feasible method for the study of pharmacokinetics and bioavailability