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目的:探讨HBV1896位终止突变株在不同HBsAg阳性乙型肝炎病人体内的检出率及与血清HBV DNA载量的关系。方法:对100份乙型肝炎病人血清,分别以EIA法、特异性引物PCR法和PCR荧光法检测HBV血清学标志物、HBV1896位终止突变株及HBV DNA载量。结果:非突变株、突变株/非突变株和突变株的检出率,在HBeAg阳性血清中分别是46.6％(27/53)、53.4％(31/58)及0%(0/58);在抗HBe阳性血清中分别是4.8％(1/21)、33%(7/21)和61.9％(13/21)。在HBeAg阳性和抗HBe阳性血清中,突变株/非突变株的HBV DNA载量无显著差异。单检出突变株的血清HBV DNA载量较非突变株和突变株/非突变株为低。结论:HBeAg阳性血清中以非突变株和突变株/非突变株为主,抗HBe阳性血清中以突变株为主,此种突变株的复制水平较之非突变株或突变株/非突变株有所下降。
OBJECTIVE: To investigate the detection rate of HBV1896 stop mutation in different HBsAg-positive hepatitis B patients and its relationship with the serum HBV DNA load. Methods: Serum of 100 patients with hepatitis B were detected by EIA method, specific primer PCR method and PCR fluorescence detection HBV serology markers, HBV1896 stop mutation and HBV DNA load. Results: The detection rates of non-mutants, mutants / non-mutants and mutants were 46.6% (27/53), 53.4% (31/58) and 0% (0/58) respectively in HBeAg-positive sera, ; 4.8% (1/21), 33% (7/21) and 61.9% (13/21) respectively in anti-HBe positive sera. There was no significant difference in HBV DNA load between mutant / non-mutant HBeAg-positive and anti-HBe-positive sera. The serum HBV DNA load of single mutant was lower than that of non-mutant and non-mutant. Conclusion: HBeAg-positive sera are mainly non-mutated and non-mutated / non-mutated, and anti-HBe-positive sera are predominantly mutants. The replication level of these mutants is higher than that of non-mutants or mutants / non-mutants Decline.