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目的检测过氧化物酶体增殖物激活受体γ(PPARγ)在涎腺腺样囊性癌高、低转移细胞系中的表达,并探讨PPARγ是否与涎腺腺样囊性癌(SACC)肺转移转移相关。方法采用Western blot和逆转录-聚合酶链反应(RT-PCR)及实时定量PCR(Quantitative Real-time PCR)方法检测肺高转移性涎腺腺样囊性癌(SACC-LM)及肺低转移性涎腺腺样囊性癌(SACC-83)细胞系中PPARγ蛋白表达和PPARγmRNA的表达水平。所得实验数据采用SPSS 16.0统计软件进行t检验。结果 PPARγ蛋白及mRNA在SACC-LM细胞系及SACC-83细胞系中均有表达,与SACC-83细胞系相比,SACC-LM细胞系中PPARγ蛋白较高表达,并且SACC-LM细胞系中PPARγmRNA表达水平是SACC-83细胞中表达水平的4.346939倍(P<0.01)。结论 PPARγ在SACC肺高低转移细胞系中的差异表达,初步证实PPARγ在SACC远处转移方面有重要作用。
Objective To detect the expression of peroxisome proliferator - activated receptor γ (PPARγ) in salivary adenoid cystic carcinoma cell lines with high and low metastasis, and to investigate whether PPARγ and salivary adenoid cystic carcinoma (SACC) lung Related to metastasis. Methods High-metastatic salivary adenoid cystic carcinoma (SACC-LM) and lung metastasis were detected by Western blot, RT-PCR and Quantitative Real-time PCR (Real-time PCR) Expression of PPARγ protein and PPARγ mRNA in human salivary adenoid cystic carcinoma (SACC-83) cell line. The experimental data obtained using SPSS 16.0 statistical software for t test. Results PPARγ protein and mRNA were expressed in SACC-LM cell line and SACC-83 cell line. Compared with SACC-83 cell line, PPARγ protein was highly expressed in SACC-LM cell line and in SACC-LM cell line The PPARγ mRNA expression level was 4.346939 times that of SACC-83 cells (P <0.01). Conclusions The differential expression of PPARγ in SACC lung cell lines with high and low metastasis may initially play an important role in the distant metastasis of SACC.