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收集12个健康人的外周血。每个人的外周血标本在35、37、39℃共培养三瓶,在黑暗处培养72 小时。收获前48小时加BudR(最终浓度为10μg/ml),收获前2小时加秋水仙素(最终浓度为0.24μg/ml)。按标准方法收获和固定细胞,空气干燥,用FPG技术染色。对每一个人在不同温度下培养的标本,检验20个第二次分裂的中期相细胞和观察100个连续的中
Collect peripheral blood from 12 healthy people. Peripheral blood samples of each person were co-cultured at 35, 37, 39 ° C for three bottles and incubated in the dark for 72 hours. BudR (final concentration 10 μg / ml) 48 hours before harvest and colchicine (final concentration 0.24 μg / ml) 2 hours before harvest. Cells were harvested and fixed by standard methods, air-dried and stained with FPG technique. For each specimen cultured at different temperatures, 20 second metaphase metaphase cells were examined and 100 consecutive