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脂肪组织主要有白色脂肪组织(WAT)和褐色脂肪组织(BAT)两种类型;WAT储存能量,BAT通过特异性表达解耦联蛋白1(uncoupling protein 1,UCP1)消耗能量产热。与BAT不同,WAT具有很强的可塑性,在寒冷刺激下,呈现褐色脂肪表型、获得褐色脂肪的产热活性(“褐色化”)。根据对冷刺激组(6℃)和对照组(28℃)小鼠的肩胛区褐色脂肪组织(iBAT)和皮下白色脂肪组织(sWAT)的RNA转录组测序分析结果,初步筛选出1个差异明显的长链非编码RNA(lncRNA-6030408B16Rik)。通过qRT-PCR检测lncRNA-6030408B16Rik在野生型小鼠的脾脏、肌肉、肾脏、十二指肠、大脑、肝脏、sWAT和iBAT中的表达;24只雄性C57BL/6野生型小鼠随机分成冷刺激组(6℃)和对照组(28℃),两组小鼠分别在6和28℃环境下处理10d,分别采集两组的iBAT和sWAT;并分离出生1d的野生型小鼠褐色前脂肪细胞,诱导分化后,收集0、1、3、5d的细胞,检测UCP1基因和lncRNA-6030408B16Rik在分化过程中的时空表达。qRT-PCR结果显示iBAT和sWAT中lncRNA-6030408B16Rik的表达量均明显高于其他组织;与对照组相比,冷刺激组iBAT中lncRNA-6030408B16Rik的表达量极显著上调(P<0.01),冷刺激组sWAT中lncRNA-6030408B16Rik的表达量显著上调(P<0.05);前脂肪细胞诱导分化的过程中,lncRNA-6030408B16Rik的表达量呈先上调后下调的趋势。lncRNA-6030408B16Rik在脂肪组织中特异性高表达。lncRNA-6030408B16Rik可能对白色脂肪组织“褐色化”及褐色前脂肪细胞的分化具有重要的调控作用。
There are two types of adipose tissue: white adipose tissue (WAT) and brown adipose tissue (BAT). WAT stores energy and BAT consumes energy to generate heat by specifically expressing uncoupling protein 1 (UCP1). Unlike BAT, WAT has a strong plasticity, a brown fat phenotype under cold stimulation, and a browning fat thermogenic activity (“browning”). According to the sequencing results of RNA transcriptome analysis of scapular brown adipose tissue (iBAT) and subcutaneous white adipose tissue (sWAT) in cold stimulated group (6 ℃) and control group (28 ℃), a difference of Of long-chain non-coding RNA (lncRNA-6030408B16Rik). Expression of lncRNA-6030408B16Rik in spleen, muscle, kidney, duodenum, brain, liver, sWAT and iBAT of wild-type mice was detected by qRT-PCR; 24 male C57BL / 6 wild type mice were randomly divided into cold stimulation (6 ℃) and control group (28 ℃). The two groups of mice were treated at 6 and 28 ℃ for 10 days respectively, and the iBAT and sWAT of the two groups were collected respectively. Wild-type brown preadipocytes After induction of differentiation, cells were collected for 0, 1, 3 and 5 days to detect the temporal and spatial expression of UCP1 gene and lncRNA-6030408B16Rik during differentiation. The qRT-PCR results showed that the expression of lncRNA-6030408B16Rik in iBAT and sWAT was significantly higher than that in other tissues. Compared with the control group, the expression of lncRNA-6030408B16Rik in iBAT of cold stimulation group was significantly increased (P <0.01) The expression of lncRNA-6030408B16Rik in group sWAT was significantly up-regulated (P <0.05). The expression of lncRNA-6030408B16Rik was down-regulated first and then down-regulated in preadipocyte differentiation. lncRNA-6030408B16Rik is highly expressed specifically in adipose tissue. lncRNA-6030408B16Rik may play an important regulatory role in the differentiation of white adipose tissue “browning” and brown preadipocytes.