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应用真彩色图象分析法,采用定量病理技术,测定了51例角结膜缘上皮性新生物(上皮良性增生、上皮非典型性增生、原位癌和鳞状细胞癌)细胞核内的DNA含量。发现,上皮良性增生病变中,二倍体～四倍体(2C-4C)细胞占88.59%,≥五倍体(≥5C)细胞仅占11.41%;上皮非典型性增生中,2C-4C细胞占58.62%,≥5C细胞占41.38;原位癌、鳞状细胞癌中,2C-4C细胞分别为21.11%和21.69%,而≥5C细胞则分别占78.89%和78.31%。经统计学分析,发现有非常显著性差异(P<0.01)。认为,应用此技术可作为辅助临床病理诊断的有效手段,并可从肿瘤的细胞生物学行为来评估患者的临床预后。
The contents of DNA in nuclei of 51 cases of epithelial keratinocytes (epithelial benign prostatic hyperplasia, epithelial atypical hyperplasia, carcinoma in situ and squamous cell carcinoma) were determined by real color image analysis and quantitative histopathology. It was found that the number of diploid to tetraploid (2C-4C) cells accounted for 88.59%, and the percentage of ≥5ploidy (≥5C) cells accounted for only 11.41%. In epithelial atypical hyperplasia, 2C-4C cells Accounting for 58.62%, ≥5C cells accounted for 41.38; in situ carcinoma, squamous cell carcinoma, 2C-4C cells were 21.11% and 21.69%, respectively, while ≥ 5C cells accounted for 78.89% and 78.31%. After statistical analysis, we found a very significant difference (P <0.01). It is believed that the application of this technique can be used as an effective method to assist clinicopathological diagnosis and evaluate the clinical prognosis of patients based on their biological behavior.