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本文应用药物诱发隐性弓形虫感染的复发,探讨了虫血症出现及宿主脑内包囊形成的规律,旨在为亚临床型弓形虫病复发的防治和诊断提供实验依据,并对包囊形成的动态进行了分析。 实验用动物为std/ddy小鼠、虫株为FUK无毒株和BEV低毒株。用Ficoll-Pague密度梯度离心法自鼠脑分离包囊腹腔感染小鼠,每周取5只小鼠血0.5ml,抗凝,同时摘取颌下淋巴结2粒及肝组织0.1g,分别轻碾成匀浆。将上述待检材料腹腔接种正常鼠,3周后检查接种鼠脑内包囊,同时用乳胶凝集试验(LAT)检测弓形虫抗体以助诊断。感染后第六周始,每日经后肢皮下注射强的松龙连用两周,用药物前后按上法分离弓形虫,并用LAT和染色试验(DT)鉴测抗体动态,同时取血清进行免疫印迹分析。包囊检查系取鼠脑组织数取每克组织包囊数。 结果见到,小鼠腹腔感染FUK株包囊后1周时,
In this paper, the drug-induced recurrence of occult Toxoplasma gondii infection was studied, and the appearance of cyst and the formation of cysts in the brain of the host were discussed. The aim was to provide experimental evidence for the prevention and diagnosis of recurrence of subclinical toxoplasmosis. The dynamics were analyzed. Experimental animals for the std / ddy mice, strains of non-FUK FUK and BEV low strains. The mice inoculated intraperitoneally were isolated from mouse brain by Ficoll-Pague density gradient centrifugation. Five mice were perfused with 0.5 ml blood per week for anticoagulation. Two submandibular lymph nodes and 0.1 g hepatic tissue were harvested, Into homogenization. The test material was inoculated intraperitoneally normal mice, 3 weeks after vaccination in mice intracerebral cysts, while latex agglutination test (LAT) Toxoplasma gondii antibodies to help diagnose. Six weeks after the start of infection, prednisolone was administered to the hindlimbs subcutaneously twice daily for 2 weeks. Toxoplasma gondii were isolated by the above method before and after the treatment. The antibody activity was detected by LAT and DT staining, and the serum was harvested for immunoblotting analysis. Cyst examination Department of rat brain tissue taken to take the number of cells per gram of tissue. The results showed that mice infected by intraperitoneal FUK 1 week after cyst,