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谷氨酸在食品、医药、日化等方面用途广泛。但是目前,检测谷氨酸的方法存在种种缺陷而不够理想。因此,一种快速检测谷氨酸的方法很重要。文章以谷氨酸为模板,邻氨基酚为功能单体。铁氰化钾为印迹电极和底液间的探针,利用循环伏安法成功制备了谷氨酸分子印迹聚合物传感器。并优化了其反应条件(pH=5.5,t=20℃,单体:模板=2.2:1,聚合时间25圈,洗脱时间=2 min,洗脱材料:硫酸)。制备的谷氨酸分子印迹电化学传感器的相对标准偏差分别为2.91%～3.39%。线性范围0.5 mmol/L～3.0 mmol/L。对实际样品进行检测,其加标回收率为87%～110%。连续洗脱32次之后电极衰减到30%以上。与其他方法进行比较,该方法有检测灵敏度高,抗干扰能力强等优点,操作简便,为连续检测谷氨酸提供了一种新的方法。
Glutamic acid is widely used in food, medicine and daily chemical industry. However, at present, the method of detecting glutamic acid has various defects and is not ideal. Therefore, a rapid method of detecting glutamate is very important. Article glutamic acid as a template, o-aminophenol as a functional monomer. Potassium ferricyanide as a probe between the electrode and the bottom of the electrode, the use of cyclic voltammetry successfully prepared glutamate molecular imprinted polymer sensor. The optimum reaction conditions were as follows: pH = 5.5, t = 20 ℃, monomer: template = 2.2: 1, polymerization time 25 and elution time = 2 min. The relative standard deviation of prepared glutamate molecular imprinted electrochemical sensors were 2.91% ~ 3.39%, respectively. The linear range of 0.5 mmol / L ~ 3.0 mmol / L. The actual samples tested, the spike recovery of 87% to 110%. The electrode decayed to more than 30% after 32 consecutive elution. Compared with other methods, this method has the advantages of high detection sensitivity, strong anti-interference ability and simple operation, which provides a new method for the continuous detection of glutamic acid.