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目的探讨p38丝裂素活化蛋白激酶(p38MAPK)在癫痫大鼠海马结构的活性变化,并观察钙调蛋白抑制剂W-7的干预作用。方法健康SD大鼠38只,随机分为,对照组、癫痫组和W-7低剂量、中剂量、高剂量组,免疫组织化学方法和免疫印记方法检测海马组织p38MAPK蛋白表达的变化。结果癫痫组p38MAPK蛋白阳性细胞表达率较对照组明显增加,W-7各剂量的组明显较对照组降低(P<0.05或P<0.01),其中W-7高剂量组降低最为明显。结论戊四氮导致癫痫大鼠海马中p38MAPK蛋白阳性细胞表达率增高,W-7对戊四氮诱导的癫痫大鼠具有保护作用,其作用与p38MAPK的蛋白表达相关。
Objective To investigate the changes of p38 mitogen-activated protein kinase (p38MAPK) in the hippocampal formation of epileptic rats and to observe the intervention effect of calmodulin inhibitor W-7. Methods Thirty-eight healthy SD rats were randomly divided into control group, epilepsy group and W-7 low dose, middle dose and high dose group, immunohistochemistry and Western blotting to detect the expression of p38MAPK in hippocampus. Results The expression of p38MAPK protein in epilepsy group was significantly higher than that in the control group. The W-7 dose groups were significantly lower than those in the control group (P <0.05 or P <0.01). The W-7 high-dose group showed the most obvious decrease. Conclusions Pentylenetetrazol can increase the expression of p38MAPK protein in the hippocampus of epileptic rats. W-7 can protect rats from epilepsy induced by pentylenetetrazol, and its effect is related to the protein expression of p38MAPK.