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目的:建立分光光度法测定水体中微量元素汞的含量。方法:在弱碱性介质中,在表面活性剂TritonX-100的存在下,汞与邻羧基苯基重氮氨基偶氮苯发生络合反应,生成红色的络合产物,利用显色反应灵敏,采用分光光度法测定,最大吸收坡长为525 nm,表观摩尔吸光系数为1.98×105L/(mol.cm),汞含量在0.01~10μg/25 ml范围符合比尔定律。结果:相关系数r=0.9998,加标回收率为97%~102%,变异系数RSD(n=6)为3.6%。结论:实验结果表明在选定的试剂浓度及优化仪器条件下能够适应实验室对水样的测定,该方法线性良好,具有操作简便,快捷,灵敏度高等优点,可用于基层实验室对水样中汞的测定。
Objective: To establish a spectrophotometric method for the determination of trace elements mercury in water. Methods: In mild alkaline medium, the complexation of mercury with o-carboxyphenyldiazoaminoazobenzene in the presence of surfactant TritonX-100 led to the formation of a red complex product, which was characterized by its good color reaction, The maximum absorption slope of 525 nm and the apparent molar absorptivity of 1.98 × 105 L / (mol.cm) were determined by spectrophotometry. Beer ’s law was obeyed in the range of 0.01-10 μg / 25 ml of mercury. Results: The correlation coefficient was 0.9998. The recoveries were 97% -102%. The coefficient of variation RSD (n = 6) was 3.6%. Conclusion: The experimental results show that the proposed method can adapt to the laboratory determination of water samples under the conditions of the selected reagent concentration and the optimized instrument. The method has good linearity, easy operation, quickness and high sensitivity. It can be used in the laboratory Determination of mercury.