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构建并筛选出能在哺乳细胞表达的重组质粒。方法：以含ＧＦＰｃＤＮＡ报告者基因的穿梭质粒ｐＧＦＴ，－１为载体，选用人巨细胞病毒启动子，采用基因重组技术构建重组质粒。结果：重组质粒及其酶切液的琼脂糖凝胶电泳图谱表明重组质粒的构建成功。结论：该研究技术路线设计合理，以构建的重组质粒为分子工具，可望建立以ＧＦＰ基因为基础的有效的基因标记系统。
Construction and screening of recombinant plasmid expression in mammalian cells. Methods: The shuttle plasmid pGFT, -1 containing GFP cDNA reporter gene was used as a vector, and the human cytomegalovirus promoter was selected. The recombinant plasmid was constructed by gene recombination technique. Results: The agarose gel electrophoresis of the recombinant plasmid and its digested solution showed that the recombinant plasmid was successfully constructed. Conclusion: The technical route of this research is reasonable. Using the constructed recombinant plasmid as a molecular tool, it is expected to establish an effective genetic marker system based on GFP gene.