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选用抗枯萎病突变体‘威廉斯突变体(Musa spp.AAA,Williams Mutant)’为试材,用RT-PCR技术从香蕉叶中克隆得到一个长为1504bp,编码501个氨基酸的苯丙氨酸解氨酶(PAL)基因cDNA序列。序列分析与其它植物PAL蛋白有较高同源性,尤其是麻疯树和柑橘属同源性高达93%。半定量PCR和酶活性测定被采用研究威廉斯突变体在接种枯萎病菌Fusarium oxysporumf.sp.cubense race4(FOC4)茎中PAL表达的变化,结果显示茎中PAL活性呈规律性变化,且均高于同期对照,与其它植物相关研究结果类似,表明PAL与香蕉抗枯萎病密切相关。
A mutant of Fusarium wilt (Williams’ mutant, Musa spp. AAA, Williams Mutant) was used as a test material to clone a phenylalanine with a length of 1504bp and encoding 501 amino acids from banana leaves by RT-PCR Lyase (PAL) gene cDNA sequence. Sequence analysis and other plant PAL protein has high homology, especially Jatropha curcas and citrus homology as high as 93%. Semi-quantitative PCR and enzyme activity assay were used to study the changes of PAL expression in the stems of Fusarium oxysporumf.sp.cubense race4 (FOC4) in Williams inoculation. The results showed that PAL activity in the stems varied regularly The same period, similar to other plant-related research results show that PAL is closely related to banana wilt resistance.