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目的观察细颗粒物(PM_(2.5))对C57BL/6雄性小鼠糖脂代谢的影响及可能的作用机制。方法将小鼠随机分为两组,实验组以气管滴注方式进行PM_(2.5)染毒,剂量为15 mg/kg BW,对照组给予相同剂量的生理盐水。每周染毒3次,连续染毒18周,观察PM_(2.5)对小鼠的亚慢性毒性。末次染毒后进行胰岛素抵抗试验(ITT)及葡萄糖耐量试验(IPGTT),检测肝脏组织和白色脂肪组织IL-6、IL-17A、MAPK、NF-κB、TNF-αmRNA的表达情况。结果 PM_(2.5)组小鼠体重为(24.976±0.571)g,与对照组的(28.452±1.520)g比较显著降低(P<0.05)。ITT和IPGTT实验发现,与对照组小鼠比较,PM_(2.5)组小鼠出现明显的胰岛素抵抗和糖耐量异常。在肝脏组织中和白色脂肪组织中,PM_(2.5)组IL-6 mRNA表达量分别为0.373±0.185和0.364±0.089,均高于对照组的0.156±0.037和0.163±0.133,差异有统计学意义(P<0.05)。在白色脂肪中,PM_(2.5)组TNF-αmRNA表达量(0.008±0.002)高于对照组(0.004±0.001),差异有统计学意义(P<0.05)。结论 PM_(2.5)暴露可能会导致机体糖耐量异常,增加机体炎症反应和胰岛素抵抗,增加糖尿病的患病风险。
Objective To observe the effects of fine particulate matter (PM_ (2.5)) on glucose and lipid metabolism in C57BL / 6 male mice and its possible mechanism. Methods The mice were randomly divided into two groups. The experimental group was administered with PM 2.5 2.5 mg / kg BW by tracheal instillation. The control group was given the same dose of normal saline. Three times weekly exposure, continuous exposure to 18 weeks, observed PM_ (2.5) of mice subchronic toxicity. After the last exposure, insulin resistance test (ITT) and glucose tolerance test (IPGTT) were performed to detect the expression of IL-6, IL-17A, MAPK, NF-κB and TNF-αmRNA in liver tissue and white adipose tissue. Results The body weight of mice in PM 2.5 group was (24.976 ± 0.571) g, which was significantly lower than that in control group (28.452 ± 1.520 g) (P <0.05). ITT and IPGTT experiments found that compared with control mice, PM_ (2.5) mice showed obvious insulin resistance and impaired glucose tolerance. In liver tissue and white adipose tissue, the expression of IL-6 mRNA in PM 2.5 was 0.373 ± 0.185 and 0.364 ± 0.089, respectively, which were significantly higher than those in control group (0.156 ± 0.037 and 0.163 ± 0.133, respectively) (P <0.05). In white adipose tissue, the expression of TNF-αmRNA in PM 2.5 group was significantly higher than that in control group (0.008 ± 0.002) (P <0.05). Conclusion Exposure to PM 2.5 exposure may lead to impaired glucose tolerance, increase inflammation and insulin resistance, and increase the risk of diabetes.