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目的建立狼毒(月腺大戟)药材的高效液相指纹图谱。方法取月腺大戟生品粉末,用甲醇超声提取制备供试品溶液。采用Welchorm-C18柱(5μm,4.6 mm×250 mm),在检测波长226 nm,柱温25℃下,以乙腈(A%)-水(B%)为流动相,流速1ml·min-1梯度洗脱,建立不同批次饮片的指纹图谱。结果初步建立了月腺大戟药材生品指纹图谱。以月腺大戟乙素峰作为对照峰,各批狼毒(月腺大戟)药材中有20个共有峰,各峰分离度良好。结论该方法具有良好的精密度、重复性、稳定性,各共有峰间分离度高,可用于狼毒(月腺大戟)药材的真伪鉴别和质量综合评价。
Objective To establish a HPLC fingerprint of Stellera chamaejasme L. Method to take moon ghd piastre crude powder, ultrasonic extraction with methanol preparation for the test solution. A gradient of 1 ml · min-1 was obtained on a Welchorm-C18 column (5 μm, 4.6 mm × 250 mm) with a detection wavelength of 226 nm and a column temperature of 25 ° C using acetonitrile (A% Elution, the establishment of different batches of fingerprints. Results The preliminary establishment of the crude product of Radix Ostreatus crude fingerprints. In order to control the peak of lunar gland Euphorbia B cells, each batch of Euphorbia (Moon gland Euphorbia) herbs in 20 common peaks, the peak resolution is good. Conclusion The method has good precision, repeatability, stability, high separation between the common peak, which can be used for the authenticity identification and quality comprehensive evaluation of Euphorbia.