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诱导姊妹染色单体互换(SCE)已证实在检测化学诱变剂时是有效的。已知一些诱变剂在很大程度上直接诱导SCE。然而,另一些化合物(例如环磷酰胺,CPM)在肝内开始代谢并转变成活性中间物以前,没有损害染色体的作用。因此,在体外诱导SCE需用鼠肝碎片(S—9)或S—9混合物的激活系统,但使用S—9混合物的培养系统是很复杂的。本文研究灌注鼠肝的CPM的活性,以简化体外诱导SCE的试验系统。
Induced sister chromatid exchange (SCE) has been shown to be effective in detecting chemical mutagens. It is known that some mutagens directly induce SCE to a large extent. However, other compounds (eg, cyclophosphamide, CPM) do not damage the chromosomes before they begin to metabolize in the liver and convert to active intermediates. Therefore, in vitro induction of SCE requires activation of a mixture of rat liver fragments (S-9) or S-9, but a culture system using S-9 mixtures is complex. This article studies the activity of CPM perfused rat liver to simplify the experimental system for inducing SCE in vitro.