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目的：研究高效液相色谱法测定中药片剂中补骨脂素和异补骨脂素的含量。方法：用反相色谱法，检测波长２４８ｎｍ；羟苯乙酯为内标；测定克白灵１号、２号片及腰痛片中的补骨脂素和异补骨脂素的含量。结果：三种片剂的回收率，补骨脂素为１０３．７％，９９．４％，１０１．５％；异补骨脂素为９９．９％，９８．０％，９６．８％。补骨脂素在４～１２０μｇ·ｍｌ－１、异补骨脂素在３～１００μｇ·ｍｌ－１浓度范围内线性关系良好，ｒ＝０．９９９９。结论：结果表明本法准确可靠，方法易行。
Objective: To study the determination of psoralen and isopsoralen in traditional Chinese medicine tablets by high performance liquid chromatography. Methods: The content of psoralen and isopsoralen in Ke Bai Ling No. 1, No. 2 tablets and low back pain tablets were determined by reverse phase chromatography, detection wavelength of 248 nm and hydroxyphenylethyl ester as internal standard. Results: The recoveries of three tablets were 103.7%, 99.4% and 101.5%, respectively. The isopsoralen was 99.9%, 98.0% and 96.8% . Psoralen at 4 ~ 120μg · ml-1, isopsoralen at a linear range of 3 ~ 100μg · ml-1 concentration, r = 0.9999. Conclusion: The results show that the method is accurate and reliable, the method is easy.