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本文以混合培养海马神经元技术为基础，通过改进解剖方法、摸索鼠龄与存活率之间的关系，成功地进行了海马神经元的分区培养。同时采用同视野跟踪记数法、激光扫描共聚焦显微镜测钙和原位杂交等技术，研究了缺氧条件下培养的海马CA1和DG神经元在存活率、细胞内游离钙和脑源性神经营养因子（BDNF）mRNA表达水平等指标上变化的差异。结果表明，在相同的缺氧条件下，DG细胞比CAl细胞损伤轻微并且具有比CAl细胞更强的维持细胞内Ca2＋稳态和表达BDNFmPNA．的能力。
Based on the mixed culture of hippocampal neurons, this study succeeded in culturing the hippocampal neurons by improving the anatomical method and exploring the relationship between the mice age and survival rate. At the same time using the same field tracking counting method, laser scanning confocal microscopy of calcium and in situ hybridization techniques to study hypoxia cultured hippocampal CA1 and DG neurons in the survival rate, intracellular free calcium and brain-derived nerve Nutritional factors (BDNF) mRNA expression levels and other indicators of changes in the difference. The results indicate that DG cells are less damaged than CAl cells under the same hypoxic conditions and have more robust intracellular Ca2 + homeostasis and BDNFmPNA expression than CA1 cells. Ability.