Cloning of HBsAg-encoded genes in different vectors and their expression in eukaryotic cells

• 作 者: ShanQin HongTang Lian-SanZhao
• 机 构: KeyLaboratoryforMolecularBiologyofInfectiousDiseasesofSichuanProvince,AnalyticalandTestingCenter,Cen
• 出 处: 世界胃肠病学杂志：英文版（电子版）
• 发表日期: 2003年5期
• 关键词: 乙肝表面抗原 编码基因 真核细胞 克隆 乙型肝炎病毒 病毒携带者
• 基金项目: 国家科学基金

AIM: To compare the efficiency of different plasmids as DNA vectors by cloning three HBsAg-encoded genes into two eukaryotic expression vectors, pRc/CMV and pSG5UTPL/Flag, and to express HBsAg S, MS, and LS proteins in SP2/0 cells, and to establish monocl