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通过离体烟叶平皿叠培养筛选模型得到的链霉菌S—10菌株所产生的农用抗生素对烟草赤星病菌具有很强的抑制作用。用牛津小杯法测定的抑菌圈直径可达320cm。对抗生素进行了抗菌谱、pH纸色谱、捷克八溶剂系统纸色谱等试验,初步鉴定该抗生素为碱性水溶性广谱抗生素。抗生素发酵液的温室盆栽试验两种处理的防治效果分别为745%、753%。
The agricultural antibiotics produced by the Streptomyces S-10 strain obtained from the in vitro culture model of the in vitro tobacco leaf bundle have a strong inhibitory effect on the brown spot pathogen. With the determination of Oxford small cup diameter of the inhibition zone up to 3 20cm. Antibiotic antibacterial spectrum, pH paper chromatography, the Czech eight solvent system paper chromatography and other tests, preliminary identification of the antibiotic as alkaline water-soluble broad-spectrum antibiotics. The control effect of the two treatments in the greenhouse pot experiment of antibiotic fermentation broth was 745% and 753%, respectively.