Cloning of C-Terminal of Opioid μ-Receptor and Construction of Its Expression Plasmid for Yeast Two

• 作 者: YANHui GONGZe-hui
• 机 构: InstituteofPharmacologyandToxicology
• 出 处: 中国药理通讯
• 发表日期: 2004年2期
• 关键词: 基因遗传 C-端点 阿片μ-受体 基因表达 细胞质粒 AHl09

Aim: To obtain the C-terminal DNA and construct the expression plasmid in yeast two-hybrid. Methods: About 177bp DNA fragment was amplified from the complete sequence of ( receptor by PCR. After being sequenced, the C-terminal fragment was ligased into Ec