【摘 要】
：
Gui-Zhi-Jia-Ge-Gen-Tang (GZJGGT) is a traditional Chinese medicine (TCM) prescription commonly used to treat cervical spondylopathy,scapulohumeral periarthritis,etc.Though it is widely applied in clinical practice,the effective constituents of GZJGGT rema
【机 构】
：
College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China;Children’s Hospital,
论文部分内容阅读
Gui-Zhi-Jia-Ge-Gen-Tang (GZJGGT) is a traditional Chinese medicine (TCM) prescription commonly used to treat cervical spondylopathy,scapulohumeral periarthritis,etc.Though it is widely applied in clinical practice,the effective constituents of GZJGGT remain unclear.This was the first report on the identification of the chemical constituents from GZJGGT in vitro and in vivo using LC-IT-MS combined with LC-Q-TOF-MS.A total of 141 constituents were detected in GZJGGT,and 77 were identified.These compounds mainly included flavonoid glycosides,triterpene saponins,monoterpene glycosides,puerosides,and organic acids.Among them,12 compounds were unequivocally identified in comparison with reference substances.Additionally,a diagnostic base peak ion filtering strategy for rapid classification of flavonoid O-glycosides and C-glycosides was proposed.After gastrointestinal administration of GZJGGT to rats,45 prototypes and 48 metabolites in rat plasma were speculated.In addition,the metabolic profile of GZJGGT was portrayed to understand interrelationship between metabolites.
其他文献
Pancreatic lipase (PL),a crucial enzyme in the digestive system of mammals,has been proven as a therapeutic target to prevent and treat obesity.The purpose of this study is to evaluate and characterize the PL inhibition activities of the major constituent
The aim of this study is to investigate the protective effects of a small molecular fraction (SMF) ofPolygoni multiflori Radix Praeparata (PMRP) in a cyclophosphamide (CTX) induced anemia mouse model.Small molecular fraction of PMRP was prepared and ident
To recognize the potential medicinal value of the Dendrobium sonia,polysaccharide (DSP) was extracted,purified,and investigated for its immunomodulatory activity.In vitro,DSP was shown to enhance the viability (MTT assay) and phagocytosis of macrophages.I
The dried roots of Berberis heteropoda Schrenk have traditionally been used to treat acute gastroenteritis and dysentery.The aim of this study was to confirm the antibacterial activity of an extract of Berberis heteropoda Schrenk root in vitro and its the
The aim is to select a universal DNA barcode for identifying all poisonous medicinal plants in Chinese pharmacopoeia and their poisonous related species or adulterants.We chose 4 commonly used regions as candidate DNA barcodes (ITS2,psbA-trnH,matK and rbc
Loureirin A is a major active component of Draconis sanguis,a traditional Chinese medicine.This work aimed to investigate the activity of loureirin A against Candida albicans biofilms.2,3-Bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilid
In an effort to understand the molecular events contributing to the cytotoxicity activity of resveratrol (RSV),we investigated its effects on human lung adenocarcinoma epithelial cell line A549 at different concentrations.Cellular nucleoside metabolic pro
Five new polyhydroxylated furostanol saponins were isolated from the roots and rhizomes of Tupistra chinensis,and their structures were determined as tupistrosides J-N (1-5),together with four known furostanol saponins (6-9),on the basis of physico-chemic
3-Hydroxy-3-methylglutaryl-CoA synthase (HMGS) is the first committed enzyme in the MVA pathway and involved in the biosynthesis of terpenes in Tripterygium wilfordii.The full-length cDNA and a 515 bp RNAi target fragment of TwHMGS were ligated into the p
Whitmania pigra has been used as a traditional Chinese medicine (TCM) for promoting blood circulation,alleviating blood coagulation,activating meridians and relieving stasis for several hundred years.However,the therapeutic components of this species,espe