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本文对30例来自妇科门诊的非性病患者和70例来自皮肤科,妇科和泌尿科门诊的疑淋病患者分别作涂片、培养、PCR检测NG和酶标法检测NG抗体四项检测,以培养为对照评价各方法的临床应用价值。结果表明,在30例非性病患者中,培养和涂片均为阴性,但PCR和NG抗体检测各有一例假阳性,假阳性率为3.3%。在70例疑淋病患者中,有15例培养阳性,其中PCR阳性为13例,故PCR的假阴性率为13.3%。因此,NG感染的确证以培养为主仍然合理可行。目前开展的淋病PCR检测,因试剂盒制备、实验条件、操作技术等因素,其敏感性和特异性有相当大的差异,故虽对培养阴性的患者可能有一定的提示作用,但应谨慎从事。NG抗体检测对急性期淋病患者无诊断价值。
In this paper, 30 cases of non-STD patients from gynecology clinic and 70 cases of suspected gonorrhea patients from dermatology, gynecology and urology clinic were smear, culture, PCR detection of NG and enzyme-labeled NG antibody detection four to culture To evaluate the clinical value of each method. The results showed that in 30 non-STD patients, both culture and smear were negative, but there was a false-positive PCR and NG antibody test with a false-positive rate of 3.3%. Among 70 cases of suspected gonorrhea, 15 cases were positive for culture, including 13 cases of PCR positive, so the false negative rate of PCR was 13.3%. Therefore, the confirmation of NG infection to culture-based still reasonable and feasible. Currently carried out gonorrhea PCR detection, due to the kit preparation, experimental conditions, operating techniques and other factors, the sensitivity and specificity of considerable differences, so although the culture of negative patients may have some tips, but should be cautious . NG antibody testing no diagnosis of acute gonorrhea patients.