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A carbon/PVC-COOH/ferrocene composite electrode used for the determination of glucose has been prepared. The ferrocene acted as mediator was incorporated into the PVC-COOH polymer and the leakage could be prevented. The presence of carboxyl groups on the electrode surface allowed immobilizing enzyme via EDC and NHS. The ratio of PVC-COOH to graphite powder (w/w) has been studied. Amperometric determination of glucose has been performed at potential of 0.30 V vs SCE. The response time was < 15 s. The linear response range was of 0.1-20 mmol/L with a detection limit of 48 μmol/L.
The ferrocene acted as mediator was incorporated into the PVC-COOH polymer and the leakage could be prevented. The presence of carboxyl groups on the electrode surface allowed The ratio of PVC-COOH to graphite powder (w / w) has been studied. Amperometric determination of glucose has been performed at a potential of 0.30 V vs SCE. The response time was <15 s. The linear response range was 0.1-20 mmol / L with a detection limit of 48 μmol / L.