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目的探讨饮酒对大鼠睾丸组织睾酮合成和雄激素结合蛋白(ABP)mRNA表达的影响。方法雄性Wistar大鼠40只,按体重随机分为4组,每组10只,即对照组(蒸馏水5g·kg~(-1)·d~(-1));大剂量饮酒组(酒精量5 g·kg~(-1)·d~(-1));中剂量饮酒组(酒精量2.5 g·kg~(-1)·d~(-1));小剂量饮酒组(酒精量0.5g·kg~(-1)·d~(-1)),喂养时间5个月。ELISA测定睾酮含量,RT-PCR测定睾九组织外周型苯二氮革受体(PBR)、PPARα和ABP mRNA水平。结果与对照组相比,(1)大剂量饮酒组大鼠睾丸组织睾酮含量下降31.13%(P<0.05),中剂量饮酒组下降26.8%(P<0.05),小剂量饮酒组下降14.2%(P>0.05);(2)各饮酒组PBR mRNA水平明显降低(均P<0.05),PPARαmRNA水平也明显降低(均P<0.05);(3)各饮酒组ABP mRNA水平明显下降(均P<0.05)。结论长期饮酒可显著降低大鼠睾酮合成,PBR和PPARα表达降低是其可能机制之一;长期饮酒还可抑制大鼠ABP表达,影响睾酮生物学效应的发挥。
Objective To investigate the effects of drinking alcohol on testosterone synthesis and the expression of androgen-binding protein (ABP) mRNA in the testis of rats. Methods Forty male Wistar rats were randomly divided into four groups according to body weight, with 10 rats in each group as the control group (distilled water, 5g · kg -1 · d -1); high-dose alcohol group 5 g · kg -1 · d -1); middle-dose drinking alcohol group (alcohol 2.5 g · kg -1 · d -1); low-dose alcohol group 0.5g · kg -1 (-1) d -1) for 5 months. The levels of testosterone were determined by ELISA. The levels of perylene-benzodiazepine receptor (PBR), PPARα and ABP mRNA in testis tissue were determined by RT-PCR. Results Compared with the control group, testosterone in testis decreased by 31.13% (P <0.05), decreased by 26.8% (P <0.05) in middle dose group and decreased by 14.2% (P <0.05) in low dose group (P <0.05); (2) The levels of PBR mRNA were significantly decreased in all drinking groups (all P <0.05) and PPARαmRNA levels were significantly lower (all P <0.05); (3) 0.05). Conclusion Long-term alcohol consumption can significantly reduce the testosterone synthesis in rats. The decrease of PBR and PPARα expression is one of the possible mechanisms. Long-term alcohol consumption can inhibit the expression of ABP and affect the testosterone biological effects.