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目的研究2012年-2013年北京市西城区食源性单核细胞增生李斯特菌的同源性和耐药情况。方法按GB4789.30—2010方法分离鉴定菌株,按照标准化的Lm-PFGE方法对分离到的13株单核细胞增生李斯特菌,进行脉冲场凝胶电泳分型,药敏试验采用K-B法和E-test法。结果单核细胞增生李斯特菌总检出率为9.85%,耐药率为46.15%,主要耐药的抗生素有环丙沙星、红霉素、氯霉素、左氧氟沙星、美罗培南和头孢噻肟,并出现了多重耐药株。13株单核细胞增生李斯特菌通过PFGE分成了6个型别,以GX6A16.CN0004为主要带型(7株),占总菌株数的53.85%。结论本城区2012年-2013年部分食品不同程度地受到单核细胞增生李斯特菌的污染,GX6A16.CN0004基因型为优势菌,2013年出现新的基因型,单核细胞增生李斯特菌对多种抗生素敏感。加强对新型基因型的监测和临床使用抗生素的管理。
Objective To study the homology and drug resistance of Listeria monocytogenes in Xicheng District, Beijing from 2012 to 2013. Methods According to the method of GB4789.30-2010, strains were isolated and identified. According to the standardized Lm-PFGE method, 13 isolates of Listeria monocytogenes were isolated and subjected to pulsed-field gel electrophoresis. The susceptibility test was performed using KB method and E -test method. Results The total detection rate of Listeria monocytogenes was 9.85%, and the resistance rate was 46.15%. The main antibiotics resistant were ciprofloxacin, erythromycin, chloramphenicol, levofloxacin, meropenem and cefotaxime , And appeared multiple drug-resistant strains. Thirteen strains of Listeria monocytogenes were divided into six types by PFGE, with GX6A16.CN0004 as the main band (7 strains), accounting for 53.85% of the total strains. Conclusion Some of the foods from 2012 to 2013 in our district were contaminated by Listeria monocytogenes to varying degrees. The genotype GX6A16.CN0004 was the predominant bacteria. In 2013, a new genotype was found in which Listeria monocytogenes was more Antibiotic sensitivity. Strengthen the monitoring of new genotypes and the clinical management of antibiotics.