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目的:研究菊科艾Artemisia argyi干燥叶的化学成分及抗乙型肝炎病毒(HBV)活性。方法:采用硅胶柱色谱分离其化学成分,并根据理化性质、核磁共振波谱法、质谱法以及参考相关文献鉴定化合物结构,采用噻唑蓝(MTT)法测定化合物对Hep G 2.2.15细胞的毒性以及酶联免疫(ELISA)法检测各组分对Hep G 2.2.15细胞上清液中HBe Ag和HBs Ag分泌的影响。结果:从艾叶乙酸乙酯部位分离鉴定了5个化合物,分别为豆甾醇(1),5,4’-二羟基-6,7,3’-三甲氧基黄酮(3’-甲氧基蓟黄素)(2),达玛二烯醇乙酸酯(3),5,7,4’-三羟基-3’,8-二甲氧基黄酮(4),十六烷酸丙酯(5)。结论:化合物2~4为首次从艾叶中分离得到,其中3’-甲氧基蓟黄素对HBs Ag和HBe Ag有抑制作用,其对HBs Ag的半数抑制浓度(IC50)为8.09 mg·L-1,对HBe Ag的IC50小于2.5 mg·L-1。
Objective: To study the chemical constituents and anti-hepatitis B virus (HBV) activity of dry leaves of Artemisia argyi. Methods: The chemical constituents were isolated by silica gel column chromatography. The structures of the compounds were identified by their physicochemical properties, nuclear magnetic resonance spectroscopy, mass spectrometry and related references. The toxicity of the compounds to Hep G 2.2.15 cells was determined by MTT assay. The effects of different components on the secretion of HBeAg and HBsAg in supernatant of Hep G 2.2.15 cells were detected by enzyme-linked immunosorbent assay (ELISA). Results: Five compounds were isolated and identified from the ethyl acetate fraction of Artemisiae rhamnoides L., which were stigmasterol (1), 5,4’-dihydroxy-6,7,3’-trimethoxyflavone Flavin (2), damaldienol acetate (3), 5,7,4’-trihydroxy-3 ’, 8-dimethoxyflavone (4), palmitic acid propyl 5). CONCLUSION: Compounds 2 ~ 4 were isolated from leaves for the first time. Among them, 3’-methoxythymidine inhibited HBsAg and HBeAg, and the IC50 of HBsAg was 8.09 mg · L -1 and the IC50 of HBe Ag is less than 2.5 mg · L -1.