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In a charged colloidal system, the influence on depletion interaction between two like-charged macro-ions is studied through Monte Carlo simulation in this paper. The numerical results show that this depletion force is affected by both the electrostatic interactions between charged spheres and charged plates and by the geometrical factor of the two charged plates, and they further indicate that the influence of geometrical confinement on the depletion interaction is larger than that of electrostatic potential.