论文部分内容阅读
目的:探讨长期游泳训练和补充大豆多肽(SPH)对高脂饮食大鼠胰岛素抵抗(IR)形成的干预作用及其机制。方法:60只雄性SD大鼠随机分成对照组、高脂饮食组、高脂饮食+SPH组、高脂饮食+运动组和高脂饮食+运动+SPH组5组。除对照组外,在给大鼠喂饲高脂饮食的同时,按分组方案分别进行10周60min的游泳训练或/和补充SPH(500mg/kgBW),实验结束时测定空腹血糖(FPG)、空腹胰岛素(FINS)、血清C肽(CP)等指标,并计算胰岛素敏感性指数(ISI)、稳态模型-IR指数(HOMA-IR)。结果:(1)10周高脂饮食后大鼠FPG、FINS、HOMA-IR显著升高,而ISI显著降低,但血清CP含量无显著性变化;(2)在高脂饮食诱导大鼠IR形成的同时,进行游泳锻炼或补充SPH,可以显著降低高脂饮食大鼠FPG、FINS、HOMA-IR,提高ISI,但对血清CP含量无显著影响,运动联合补充SPH可使高脂饮食大鼠FINS、HOMA-IR进一步降低,ISI进一步升高,但无显著性差异,同时使血清CP含量显著升高。结论:(1)10周高脂饮食可以诱导大鼠IR形成,这可能与高脂饮食大鼠胰岛素敏感性(IS)降低有关;(2)运动训练或补充SPH可以通过改善机体IS预防高脂饮食大鼠IR的形成。但运动训练联合补充SPH能否对预防高脂饮食大鼠IR的形成具有协同促进作用还有待于进一步研究。
AIM: To investigate the effects of long-term swimming training and supplementation of soybean peptide (SPH) on insulin resistance (IR) in rats with high fat diet and its mechanism. Methods: Sixty male Sprague Dawley rats were randomly divided into five groups: control group, high fat diet group, high fat diet + SPH group, high fat diet + exercise group and high fat diet + exercise + SPH group. In addition to the control group, rats were fed a high-fat diet at the same time, according to the grouping program 60 weeks of 10 weeks of swimming training or / and SPH supplementation (500mg / kgBW), the end of the experiment measured fasting blood glucose (FPG) Insulin (FINS) and serum C-peptide (CP), and insulin sensitivity index (ISI) and homeostasis model index (HOMA-IR) were calculated. Results: (1) FPG, FINS and HOMA-IR of rats after 10 weeks of high-fat diet increased significantly, but ISI decreased significantly, but there was no significant change of serum CP. (2) IR induced by high-fat diet At the same time, swimming exercise or SPH supplementation significantly reduced FPG, FINS, HOMA-IR and ISI in high-fat diet rats, but had no significant effect on serum CP levels. Exercise combined with SPH significantly reduced FINS , HOMA-IR further reduced, ISI further increased, but no significant difference, while significantly increased serum CP levels. Conclusion: (1) 10-week high-fat diet can induce IR formation in rats, which may be related to the decrease of insulin sensitivity (IS) in rats fed high-fat diet. (2) Exercise training or SPH supplementation can improve the body fat Formation of dietary IR in rats. However, exercise training combined with SPH can prevent the formation of IR in rats with high-fat diet has a synergistic effect remains to be further studied.