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AIM:To investigate the effects of Terminalia arjuna(T.arjuna)extract on human hepatoma cell line(HepG2)and its possible role in induction of apoptosis.METHODS:Human hepatoma cells were treated withdifferent concentrations of ethanolic extract of T.arjunaand its cytotoxicity effect was measured by trypan blueexclusion method and lactate dehydrogenase leakageassay.Apoptosis was analyzed by light and fluorescencemicroscopic methods,and DNA fragmentation.Themechanism of apoptosis was studied with expressionof p53 and caspase-3 proteins.Glutathione(GSH)content was also measured in HepG2 cells after T.arjunatreatment.RESULTS:T.arjuna inhibited the proliferation of HepG2cells in a concentration-dependent manner.Apoptoticmorphology was observed in HepG2 cells treated with T.arjuna at the concentrations of 60 and 100 mg/L.DNAfragmentation,accumulation of p53 and cleavage ofprocaspase-3 protein were observed in HepG2 cells afterthe treatment with T.arjuna.The depletion of GSH wasobserved in HepG2 cells treated with T.arjuna.CONCLUSION:T.arjuna induced cytotoxicity in HepG2cells in vitro.Apoptosis of HepG2 cells may be due tothe DNA damage and expression of apoptotic proteins.Depletion of GSH may be involved in the induction ofapoptosis of HepG2 cells.
AIM: To investigate the effects of Terminalia arjuna (T.arjuna) extract on human hepatoma cell line (HepG2) and its possible role in induction of apoptosis. METHODS: Human hepatoma cells were treated with different concentrations of ethanolic extract of T. jarjuna and its cytotoxicity effect was measured by trypan blueexclusion method and lactate dehydrogenase leakageassay. Apoptosis was analyzed by light and fluorescence microscopic methods, and DNA fragmentation. The mechanism of apoptosis was studied with expression of p53 and caspase-3 proteins. Glutathione (GSH) content was also measured in HepG2 cells after T. arjunatreatment.RESULTS: T.arjuna inhibited the proliferation of HepG2 cells in a concentration- dependent manner .poppopology was observed in HepG2 cells treated with T.arjuna at the concentrations of 60 and 100 mg / L.DNAfragmentation, accumulation of p53 and cleavage ofprocaspase-3 protein were observed in HepG2 cells afterthe treatment with T.arjuna. depletion of GSH wasobserved in HepG 2 cells treated with T. arjuna. CONCLUSION: T.arjuna induced cytotoxicity in HepG2 cells in vitro. Apoptosis of HepG2 cells may be due to DNA damage and expression of apoptotic proteins. Chapter of the GSH may be involved in the induction of apoptosis of HepG2 cells.