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目的:研究六月青皂苷(LYQS)对四氯化碳诱导大鼠肝损伤的保护作用。方法:66只SD大鼠,分为两组,正常组(10只)和造模组(56只),造模组大鼠,采用50%CCl4花生油溶液1 mL·kg-1,ig造模,每周2次,连续6周,正常组用生理盐水代替。随机抽取造模组6只大鼠进行肝脏病理检查,确认肝损伤形成后,将50只SD大鼠随机分为:模型组、秋水仙碱组、LYQS低、中、高剂量组。ig六月青皂苷20,40,80 mg·kg-1,秋水仙碱0.2 mg·kg-1,正常组及模型组给予等量生理盐水,每天ig给药1次,连续ig 30 d。末次给药24 h后取材,检测大鼠血清中丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、肿瘤坏死因子-α(TNF-α)、白介素-6(IL-6)的含量。同时Western blot法测定肝组织中环加氧酶2(COX-2)的表达,并观察肝组织病理学变化。结果:与模型组ALT,AST,TNF-α,IL-6依次为(91.62±4.84)U.L-1,(98.56±9.37)U.L-1,(135.64±5.38)ng·L-1,(147.38±6.34)ng·L-1比较,六月青皂苷低、中、高给药组均有效降低模型大鼠血清中ALT(80.94±4.68),(69.51±4.07),(65.89±3.85)U.L-1,AST(90.87±8.76),(81.53±6.62),(73.64±5.71)U.L-1,TNF-α(98.57±3.09),(81.74±4.33),(65.93±3.20)ng·L-1,IL-6(115.78±4.81),(95.83±5.34),(74.29±4.76)ng·L-1水平(P<0.05);治疗后,与模型组(1.18±0.17)比较,六月青皂苷低、中、高给药组的肝组织中COX-2蛋白水平(0.91±0.03),(0.79±0.06),(0.67±0.04)均有所降低(P<0.05),并缓解肝损伤病情。结论:六月青皂苷对CCl4诱导的大鼠肝损伤具有一定保护作用,其机制可能与其抗炎作用有关。
Objective: To study the protective effect of LYQS on carbon tetrachloride-induced hepatic injury in rats. Methods: Sixty-six SD rats were divided into two groups: normal group (10 rats) and model group (56 rats). Rats in model group were treated with 50% CCl4 peanut oil solution (1 mL · kg- , Twice a week for 6 weeks, the normal group with saline instead. Six rats in the model group were randomly selected for liver pathology examination. After liver injury was confirmed, 50 SD rats were randomly divided into model group, colchicine group, LYQS low, medium and high dose groups. ig June saponin 20,40,80 mg · kg-1, colchicine 0.2 mg · kg-1, the normal group and model group were given the same amount of saline, ig administration once a day, continuous ig 30 d. Twenty-four hours after the last administration, the contents of ALT, AST, TNF-α, IL-6 content. At the same time, Western blot was used to detect the expression of cyclooxygenase 2 (COX-2) in liver tissue and the pathological changes of liver were observed. Results: Compared with the model group, the levels of ALT, AST, TNF-α and IL-6 were 91.62 ± 4.84 UL-1, 98.56 ± 9.37 UL-1, 135.64 ± 5.38 ng · L-1 and 147.38 ± 6.34) ng · L-1, the low, middle and high doses of the group treated with both of the six saponins were effective in reducing the serum levels of ALT (80.94 ± 4.68), (69.51 ± 4.07) and (65.89 ± 3.85) UL-1 , AST (90.87 ± 8.76), (81.53 ± 6.62), (73.64 ± 5.71) UL-1, TNF-α (115.78 ± 4.81), (95.83 ± 5.34) and (74.29 ± 4.76) ng · L -1, respectively (P <0.05). Compared with the model group (1.18 ± 0.17) The levels of COX-2 protein in the middle and high dose groups were significantly decreased (0.91 ± 0.03), (0.79 ± 0.06) and (0.67 ± 0.04), respectively (P <0.05), and alleviated the liver injury. CONCLUSION: Junina saponin has a protective effect on CCl4-induced hepatic injury in rats, and its mechanism may be related to its anti-inflammatory effects.