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目的:建立同时测定药用明胶硬空心胶囊中9种β-受体激动药残留量的超高效液相-串联四级杆质谱方法(UPLCMS/MS)。方法:以多反应监测模式测定,以Waters ACQUITY UPLC~ BEH C_(18)柱进行分离,乙腈(含0.1%甲酸)-0.1%甲酸溶液梯度洗脱,流速:0.3 ml·min~(-1),离子化模式:ESI~+。结果:9种β-受体激动药线性范围0.25～5.00μg·kg~(-1),r为0.994 3～0.998 1;方法的最低检出浓度0.010～0.086μg·kg~(-1),回收率为70.01%～1 13.72%。结论:该方法快速、准确、灵敏,可作为同时测定药用明胶硬空心胶囊壳中9种β-受体激动药残留的方法。
OBJECTIVE: To establish an ultra performance liquid chromatography-tandem quadrupole mass spectrometry (UPLC MS / MS) method for the simultaneous determination of nine β-agonists residues in medicinal hard hollow capsules. Methods: The method was separated on a Waters ACQUITY UPLC ~ BEH C 18 column and eluted with a gradient of acetonitrile 0.1% formic acid 0.1% formic acid at a flow rate of 0.3 ml · min -1 ), Ionization mode: ESI ~ +. Results: The linear range of 9 β-agonists was 0.25-5.00 μg · kg -1, r was 0.994 3-0.998 1, and the lowest detection limit was 0.010-0.086 μg · kg -1. The recoveries ranged from 70.01% to 13.72%. Conclusion: The method is rapid, accurate and sensitive and can be used as a method for the simultaneous determination of nine β-agonists in hard hollow capsules.