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目的体外研究姜黄素对丙烯酰胺(AA)所致人肝癌HepG2细胞DNA损伤的保护作用。方法AA(0、2.5、5.0、10.0和20.0mmol/L)与HepG2细胞温育1h,单细胞凝胶电泳检测DNA损伤;姜黄素(0.63、1.25和2.50μg/ml)37℃预处理HepG2细胞1h后,再与不同浓度的AA(0、10和20mmol/L)温育1h,单细胞凝胶电泳检测DNA损伤,2’,7’-二氢二氯荧光比色法测定细胞内活性氧水平。结果2.5、5.0、10.0和20.0mmol/LAA组的彗尾DNA含量、彗星尾长及彗星尾矩均显著高于对照组(P<0.05),姜黄素保护组的彗尾DNA含量、彗星尾长及彗星尾矩均比相同浓度AA处理组降低,2.50μg/ml姜黄素保护组的差异有显著性(P<0.05);AA(10和20mmol/L)作用于HepG2细胞1h后,细胞内ROS水平显著升高(P<0.01),2.50μg/ml姜黄素预处理组ROS水平显著低于单独AA处理组(P<0.01)。结论姜黄素对AA所致HepG2细胞DNA损伤具有保护作用。
Objective To study the protective effect of curcumin on DNA damage induced by acrylamide (AA) in human hepatocellular carcinoma HepG2 cells in vitro. Methods HepG2 cells were incubated with HepG2 cells (0, 2.5, 5.0, 10.0 and 20.0 mmol / L) for 1 h. DNA damage was detected by single cell gel electrophoresis. HepG2 cells were pretreated with curcumin at 0.63,1.25 and 2.50 μg / 1h, then incubated with different concentrations of AA (0, 10 and 20mmol / L) for 1h, single cell gel electrophoresis was used to detect DNA damage, 2 ’, 7’-dihydro dichloride fluorescent colorimetric determination of intracellular reactive oxygen species Level. Results The DNA contents of comet tail, tail length and comet tail of 2.5, 5.0, 10.0 and 20.0 mmol / LAA groups were significantly higher than those of the control group (P <0.05). The DNA content of comet tail, tail length (P <0.05). After treated with AA (10 and 20 mmol / L) for 1 h, the expression of intracellular ROS (P <0.01). The ROS level in curcumin pretreatment group was significantly lower than that in AA alone group (P <0.01). Conclusion Curcumin has a protective effect on DNA damage induced by AA in HepG2 cells.