论文部分内容阅读
小泡运输介导的先天免疫在植物防卫中起重要作用。采用定量PCR和生物信息学的方法,该研究揭示两种不同的小泡运输类型分别在花生黄曲霉抗性品种C20R和敏感品种TFR发育的种子中起主要作用。VAMP726和RMR是黄曲霉抗性品种C20R中主要的小泡运输组分,VSRs VTI1a,b是黄曲霉敏感品种TFR中主要的小泡运输组分。在果实发育过程中,这些小泡运输组分的转录动态在整体转录组水平分别与相应的花生黄曲霉抗性品种C20R和敏感品种TFR差异表达的一系列基因表达趋势一致。因此,我们认为两类不同组合的小泡运输分别在黄曲霉抗性品种C20R和敏感品种TFR果实发育中起着主要运输作用,与发育中转录组水平基因表达的差异一致。这种差异早在蛋白质合成结束和运输起始阶段就已经显示,导致果实代谢和发育方向的差异,造就黄曲霉抗性的不同。
Vesicular transport-mediated innate immunity plays an important role in plant defense. Using quantitative PCR and bioinformatics methods, the study revealed that two different vesicle trafficking types play major roles in seeds of A. peanum resistance cultivar C20R and susceptible cultivar TFR, respectively. VAMP726 and RMR are the major vesicle transport components in Aspergillus flavus-resistant variety C20R. VSRs VTI1a and b are the major vesicle transport components in TFR of Aspergillus flavus-susceptible cultivar. During fruit development, the transcriptional dynamics of these vesicle transport components at the overall transcriptome level were in line with a series of gene expression differentially expressed by the corresponding A. peanum resistant variety C20R and the susceptible TFR. Therefore, we consider that vesicular transport in two different combinations plays a major role in transport of Aspergillus flavus-resistant cultivars C20R and susceptible cultivar TFR, respectively, and is consistent with the differences in gene expression at the transcriptome level. This difference has long been demonstrated at the end of protein synthesis and in the initial stages of transport, resulting in differences in the direction of fruit metabolism and development, resulting in differences in Aspergillus flavus resistance.