论文部分内容阅读
Objectives:The purpose of this work was to study the infection course and cross-protection in mice after intradermal injection of Vaccinia virus(VACV) strain Western Reserve and three Brazilian VACV strains: Aracatuba,Muriae and BeAn58058 isolated from cow,human and rodent,respectively.Methods:Balb/c mice were inoculated by footpad and back scarification and daily monitored regarding lesion development and weight loss.To check cross protection after intradermal VACV inoculation,mice were subsequendy infected with different VACV strains and monitored to check lesion development.Serum neutralization assays were performed to check for the presence of antibodies against Orthopoxvirus.Results:After VACV intradermal inoculation the lesion development pattern was similar in mice infected with the different virus strains.By using the footpad scarification model,cross-protection among VACV strains was observed.Moreover,neutralizing antibodies against Orthopoxvirus were detected in sera from mice infected with all VACV strains.Conclusion:Although it was not possible to observe virulence differences among VACV strains isolated from cow,rodent and human using the murine model,this inoculation route showed to be an appropriated model to study lesions development since it mimics natural infections by VACV in nature.
Objectives: The purpose of this work was to study the infection course and cross-protection in mice after intradermal injection of Vaccinia virus (VACV) strain Western Reserve and three Brazilian VACV strains: Aracatuba, Muriae and BeAn58058 isolated from cow, human and rodent, respectively. Methods: Balb / c mice were inoculated by footpad and back scarification and daily monitored regarding lesion development and weight loss. Check of cross protection after intradermal VACV inoculation, mice were subsequentially infected with different VACV strains and monitored to check lesion development. neutralization assays were performed to check for the presence of antibodies against Orthopoxvirus. Results: After VACV intradermal inoculation the lesion development pattern was similar in mice infected with the different virus. By using the footpad scarification model, cross-protection among VACV strains was observed .Moreover, neutralizing antibodies against Orthopox virus were detected in sera from mic e infected with all VACV ..Conclusion: Although it was not possible to observe virulence differences among among VACV strains isolated from cow, rodent and human using the murine model, this inoculation route showed to be an appropriated model to study lesions development since it mimics natural infections by VACV in nature.