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目的:从基因组全局性角度研究E2F1对包括离子通道和G蛋白偶联受体在内的重要的跨膜信号转导基因的调控作用。方法:对从TRED和IUPHAR获取的E2F1靶基因数据和基因组离子通道及G蛋白偶联受体基因数据进行数据联配,获取E2F1调控的离子通道基因(ICG)和G蛋白偶联受体基因,并对调控基因进行家族富集性分析和组织特异性分析。结果:发现E2F1对7个ICG具有调控作用,且具有钾离子通道富集性,调控的离子通道基因具有心脏、脑、消化系统组织表达特异性。获得的11个受E2F1调控的G蛋白偶联受体基因家族富集性不明显,组织特异性表达不一致。结论:E2F1可能通过对钾离子通道基因的表达调控,实现对相应组织的作用机理影响,相应调控作用的紊乱也将导致心脏、脑或消化系统疾病,但难以确定E2F1对GPCR的调控作用效果。
OBJECTIVE: To study the regulatory effect of E2F1 on important transmembrane signal transduction genes including ion channels and G-protein coupled receptors from the perspective of genome. METHODS: E2F1 target gene data obtained from TRED and IUPHAR were genomically matched with genomic ion channel and G protein-coupled receptor gene data to obtain E2F1-regulated ion channel gene (ICG) and G protein-coupled receptor gene, We also performed family enrichment analysis and tissue-specific analysis of regulatory genes. Results: E2F1 was found to be involved in the regulation of seven ICGs, and was enriched in K + channels. The regulated ion channel genes had specific expression in heart, brain and digestive system. The eleven E2F1-regulated G protein-coupled receptor gene families were not significantly enriched and tissue-specific expression was inconsistent. Conclusion: E2F1 may affect the mechanism of action of corresponding tissues by regulating the expression of potassium ion channel genes. The disorder of corresponding regulation may lead to heart, brain or digestive diseases, but it is difficult to determine the effect of E2F1 on the regulation of GPCR.