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研究新乌头碱在大鼠尿液中的主要代谢产物。大鼠灌胃给予新乌头碱,连续收集尿液24h,尿液经固相萃取法富集纯化后,以液相色谱-电喷雾多级串联质谱法分析尿液中新乌头碱的代谢产物。在大鼠尿液中发现了新乌头碱原形药物和5个代谢产物,分别推测为新乌头次碱葡糖醛酸结合物、10-羟基新乌头碱、1-O-去甲基新乌头碱、去氧新乌头碱、新乌头次碱。在新乌头碱的5个代谢产物中,新乌头次碱葡糖醛酸结合物(m/z766)为首次发现新的新乌头碱在大鼠体内的II相代谢产物,揭示了新乌头碱在大鼠体内代谢新途径。
To study the main metabolites of mesaconitine in rat urine. Rats were given neveraconal by intragastric administration, urine was continuously collected for 24 hours. Urine was purified by solid-phase extraction and purified by liquid chromatography-electrospray ionization mass spectrometry. product. In the urine of rats found mesaconitine prototype drugs and five metabolites were speculated as new aconitine glucuronic acid conjugate, 10-hydroxy mesaconitine, 1-O-demethyl Mesaconitine, deoxy mesaconitine, Mesothelioma. Among the five metabolite of mesaconitine, the new aconitine glucuronic acid conjugate (m / z766) was the first to discover the new phase II metabolite of mesaconine in rats, revealing new A new way of aconitine metabolism in rats.