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目的:探讨不同频率中等负荷跑台训练对D-半乳糖致衰老大鼠心肌形态结构及生化指标的影响。方法:2月龄大鼠40只,随机分为正常对照组(NC组)、衰老模型对照组(AC组)、衰老模型训练Ⅰ组(AEⅠ组)和衰老模型训练Ⅱ组(AEⅡ组),每组10只。AC组、AEⅠ组和AEⅡ组每日在颈背皮下注射一次D-半乳糖(125 mg/kg)。AC组常规喂养,不训练;AEⅠ组和AEⅡ组采用坡度为零的跑台训练,第1周每天运动15 min,以后每周增加5 min,直至第6周每天40 min。AEⅠ组每周隔天训练,AEⅡ组每周训练6天,周日休息。6周后,测定各组大鼠心肌丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性,光镜观察心肌结构。结果:AEⅠ组和AEⅡ组大鼠心肌SOD活性显著高于AC组,MDA含量显著低于AC组(P<0.01);AEⅡ组心肌MDA含量显著高于NC组(P<0.01)。光镜结果显示:NC组大鼠单位面积心肌纤维面积百分比最大,AC组最小,AEⅠ组大鼠心肌纤维面积百分比大于AEⅡ组,心肌纤维数量较多,排列整齐;胞核清晰,嵴排列整齐规则且密集。结论:不同频率中等负荷运动有效改善D-半乳糖致衰老大鼠心肌结构,提高心肌抗氧化能力。
Objective: To investigate the effects of treadmill training at different intensities on myocardial morphological structure and biochemical parameters of D-galactose-induced aging rats. Methods: Forty two-month-old rats were randomly divided into normal control group (NC group), aging model control group (AC group), aging model training group Ⅰ (AEⅠgroup) and aging model training group Ⅱ (AEⅡgroup) Each group of 10. AC, AEⅠ and AEⅡ groups were injected subcutaneously with D-galactose (125 mg / kg) once daily on the dorsal neck. The rats in AC group were fed routinely and were not trained. The treadmill training was performed in AEⅠ and AEⅡ groups with zero gradient. The rats were trained for 15 min in the first week and then increased for 5 min every week until 40 min in the sixth week. The AEⅠ group was trained every other day, and the AEⅡ group was trained for 6 days a week and closed on Sunday. Six weeks later, the content of MDA and the activity of superoxide dismutase (SOD) in myocardium of rats in each group were measured. The myocardial structure was observed by light microscope. Results: Compared with AC group, the activity of SOD in myocardium of AEⅠgroup and AEⅡgroup was significantly higher than that of AC group (P <0.01). The content of MDA in AE Ⅱ group was significantly higher than that in NC group (P <0.01). The results of light microscope showed that the percentage of myocardial fiber area per unit area was the largest in NC group and the smallest in AC group. The percentage of myocardial fiber area in AE Ⅰ group was higher than that in AE Ⅱ group, and the number of myocardial fibers was more and arranged neatly. And dense. Conclusion: Moderate-load exercise at different frequencies can effectively improve the myocardial structure of D-galactose-induced senile rats and increase myocardial anti-oxidative capacity.