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在植物系统获得性抗性(SAR)中,NPR1蛋白是水杨酸介导的基因表达中关键调控因子。本研究以青农2号为试验材料,利用同源序列法和RACE技术分离甘薯SAR途径的主要抗病信号元件NPR1(none expresser of PR gene)的全长cDNA序列。序列分析表明,IbNPR1基因全长2353bp,包含一个编码586个氨基酸残基的开放阅读框,包含有类似拟南芥NPR1蛋白中的BTB/POZ和锚蛋白重复氨基酸序列结构域。聚类分析显示IbNPR1与来源于番茄的NPR1蛋白关系最近。Southern杂交及半定量RT-PCR分析表明,甘薯NPR1基因属于低拷贝基因家族,表达模式为组成型表达,并且SA能提高其表达水平。由该结果推测,IbNPR1可能在甘薯抵御病原物的侵染中起重要的作用。
In plant-derived resistance (SAR), NPR1 protein is a key regulator of salicylic acid-mediated gene expression. In this study, Qingnong 2 was used as the experimental material to isolate the full-length cDNA sequence of the major resistance signal element NPR1 (none expresser of PR gene) in sweet potato SAR by homologous sequence and RACE techniques. Sequence analysis showed that the full length of IbNPR1 gene was 2353bp, containing an open reading frame of 586 amino acid residues containing similar BTB / POZ and ankyrin repeat amino acid sequence domains in Arabidopsis NPR1 protein. Cluster analysis showed that IbNPR1 has the closest relationship with NPR1 protein derived from tomato. Southern hybridization and semi-quantitative RT-PCR analysis showed that sweet potato NPR1 gene belongs to low copy gene family, the expression pattern is constitutive expression, and SA can increase its expression level. From this result, it is speculated that IbNPR1 may play an important role in the resistance of sweet potatoes to pathogen infection.