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为探讨常规氧疗时氧气加湿和输送过程中微生物的传播规律,应用常规入水湿化和仿生表面湿化两种装置,使用大肠杆菌[CMCC(B)44102]人为污染湿源物质后,对吸氧管终端氧气中的大肠杆菌进行培养计数。湿源物质污染大肠杆菌至2.5×106cfu/ml后1、2、3d,经过入水湿化,终端氧气中大肠杆菌计数分别为26.5、55.2、65.2cfu/ml。湿源物质污染大肠杆菌至5×105cfu/ml后1、2、3d,经过入水湿化,终端氧气中大肠杆菌计数分别为12.9,29.4,37.3cfu/ml。相同条件下,经过表面湿化后,终端氧气中未检测到大肠杆菌。氧气经过表面湿化与入水湿化相比,同一时点终端氧气中的大肠杆菌计数差异均有统计学意义(P<0.01)。提示经过表面湿化,湿源物质中的大肠杆菌不能传播到吸氧管终端,而经过常规入水湿化后,大肠杆菌可以被传播到吸氧管终端。
In order to investigate the regularity of microorganism propagation in oxygen humidification and transport during routine oxygen therapy, two kinds of devices such as conventional water wetting and biomimetic surface wetting were applied. After using E. coli [CMCC (B) 44102] to pollute wet sources, Oxygen tube end of the oxygen in the culture of E. coli count. Escherichia coli was contaminated by the wet material to 2.5 × 106 cfu / ml for 1, 2 and 3 days, after which the count of Escherichia coli in the terminal oxygen was 26.5, 55.2 and 65.2 cfu / ml, respectively. Escherichia coli was contaminated by humid source to 1 × 105cfu / ml for 1, 2 and 3 days. After humidification, the count of Escherichia coli in terminal oxygen were 12.9, 29.4 and 37.3 cfu / ml, respectively. Under the same conditions, no E. coli was detected in the terminal oxygen after surface wetting. Oxygen after the surface wetting and water wetting compared to the same time point terminal oxygen in the E. coli count differences were statistically significant (P <0.01). Tip After surface wetting, the wet material in Escherichia coli can not be transmitted to the end of the oxygen tube, and after regular water humidification, Escherichia coli can be spread to the end of the oxygen tube.