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[Objective]To develop a method for simultaneously determining multi-components(i.e.,polydatin,resverotol,emodin-8-O-β-D-glucopyranosid,emodin,chrysophanol and physcion)in RHIZOMA POLYGONI CUSPIDATI and to assess the quality of RHIZOMA POLYGONI CUSPIDATI growing in different locations.[Method]The HPLC separation was performed on a reversed-phase C 18 Hyperrsil column(250 mm×4.6 mm, 5μm)with a gradient solvent system of acetonitrile-1%aqueous phosphoric acid as mobile phase(20%-38%of acetonitrile at 0-30 min and 38%-100%at 30-70 min).The flow rate was 1.0 ml/min,and the detection wavelength was 284 nm.[Result]All calibration curves of the six components showed good linearity within a certain concentration range.The recoveries(n=5)were in the range of 95.00%to 105.00%.[Conclusion]The method is simple,accurate and reliable and not only enables the simultaneous determination of the content of the stilbenes and anthraquinones in RHIZOMA POLYGONI CUSPIDATI,but also provides a simple,feasible and characteristic quality evaluation method for the Chinese herb RHIZOMA POLYGONI CUSPIDATI.
[Objective] To develop a method for the determination of multi-components (ie, polydatin, resverotol, emodin-8-O-β-D-glucopyranosid, emodin, chrysophanol and physcion) in RHIZOMA POLYGONI CUSPIDATI and to assess the quality of RHIZOMA POLYGONI CUSPIDATI growing in different locations. [Method] The HPLC separation was performed on a reversed-phase C 18 Hyperrsil column (250 mm × 4.6 mm, 5 μm) with a gradient solvent system of acetonitrile- 1% aqueous phosphoric acid as mobile phase % -38% of acetonitrile at 0-30 min and 38% -100% at 30-70 min. The flow rate was 1.0 ml / min, and the detection wavelength was 284 nm. [Result] All calibration curves of the six components showed good linearity within a certain concentration range. The recoveries (n = 5) were in the range of 95.00% to 105.00%. [Conclusion] The method is simple, accurate and reliable and not only able the simultaneous determination of the content of the stilbenes and anthraquinones in RHIZOMA POLYGONI CUSPIDATI, but also provides a simple, feasib le and characteristic quality evaluation method for the Chinese herb RHIZOMA POLYGONI CUSPIDATI.