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目的:检测TNF-α,NF-κBmRNA在病毒性肝炎中的表达以及与肝细胞凋亡的关系.方法:采用免疫组织化学方法、原位杂交技术以及原位末端标记技术(TUNEL实验)检测65例病毒性肝炎患者和10例非肝病对照者肝组织中TNF-α,NF-κBmRNA的表达和肝细胞凋亡的情况.结果:病毒性肝炎患者肝组织中TNF-α,NF-κBmRNA阳性表达率分别为76.9%和70.3%,明显高于对照组的20.0%和10.0%(P<0.01);TNF-α和NF-κBmRNA的表达与肝细胞凋亡密切相关(rs=0.267和rs=0.362,P<0.05);病毒性肝炎患者肝组织中TNF-α,NF-κBmRNA的表达存在明显的相关性(rs=0.385,P<0.05).随着慢性肝炎病理损伤的程度的加重,NF-κBmRNA的阳性表达率增高(P<0.05).结论:TNF-α,NF-κBmRNA的表达在病毒性肝炎的发病及肝细胞凋亡的发生中存在相关性.TNF-α,NF-αBmRNA的表达随着病变程度的加重而增高.
Objective: To detect the expression of TNF-α and NF-κB mRNA in viral hepatitis and its relationship with hepatocyte apoptosis.Methods: Immunohistochemistry, in situ hybridization and TUNEL were used to detect the expression of TNF-α and NF- TNF-α, NF-κB mRNA and hepatocyte apoptosis in liver tissue of patients with viral hepatitis and 10 non-liver disease controls.Results: The positive expression of TNF-α and NF-κB in hepatic tissue of patients with viral hepatitis The rates of TNF-α and NF-κB were closely related to hepatocellular apoptosis (rs = 0.267 and rs = 0.362, P <0.01) , P <0.05). There was a significant correlation between the expression of TNF-αand NF-κB mRNA in liver tissues of patients with viral hepatitis (rs = 0.385, P <0.05) .With the severity of chronic hepatitis, the expression of NF- κB mRNA (P <0.05) .Conclusion: The expression of TNF-α and NF-κB mRNA may play an important role in the pathogenesis of viral hepatitis and the occurrence of hepatocellular apoptosis.The expressions of TNF-α and NF- With the severity of the disease increased.