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目的:鉴定在南摩拉维亚结核病的可疑家庭爆发,该地区是捷克共和国内报告率最低的地区 方法:对收集到的具有同姓和/或同住所的17个本地排菌者的分离物,进行以插入序列IS6110为基础的结核分支杆菌限制性片段长度多态性分析(RFLP)。指纹谱与来自边远地区的捷克结核分支杆菌分离物的184个RFLP的数据库相匹配。 结果:RFLP分析揭示了由2-6个家庭成员或亲属组成的6次本地的爆发中的每一次部具有一致的指纹。他们中有3个群落不能与在捷克指纹库里已被发现的病例相匹配。其余3个与13个来自边远地区的捷克结核分支杆菌株的RFLP外形相一致:5个病人居住中等距离地区,8个来自远距离的地区。 结论:在具有流行病学关联的结核病家庭爆发中,确定了结核分支杆菌不同群落的基因型,他们中一半是本地的,另一半与来自偏远地区的菌株指纹相匹配。似乎在南摩拉维亚不断发生的结核病家庭爆发可能因为该地区是在捷克共和国中报告率最低的。
Purpose: To identify the outbreak of suspicious families of tuberculosis in South Moravia, the least-regional method of reporting in the Czech Republic: for isolates of 17 indigenous bacteriolytes collected with the same surname and / or cohabitation, Mycobacterium tuberculosis restriction fragment length polymorphism analysis (RFLP) based on the insert sequence IS6110 was performed. Fingerprinting spectra matched 184 RFLP databases from Czech Mycobacterium isolates from remote locations. Results: The RFLP analysis revealed that each of the six local outbreaks consisting of 2-6 family members or relatives had consistent fingerprints. Three of them can not match the cases that have been found in the Czech fingerprint database. The remaining three were consistent with the RFLP profiles of 13 Czech Mycobacterium tuberculosis isolates from remote areas: 5 patients residing in the medium-distance area and 8 from remote areas. CONCLUSIONS: In outbreaks of epidemic-linked tuberculosis families, the genotypes of different M. tuberculosis isolates were identified, half of them local and the other half matched strains of fingerprints from remote areas. It appears that the ongoing outbreak of TB families in South Moravia may be the lowest reported in the Czech Republic.