Cloning and sequencing of nifBHDKENX genes of Paenibacillus massiliensis T7 and its nif promoter ana

• 作 者: ZHAO Hongxin XIE Baoen CHEN Sa
• 机 构: StateKeyLaboratoryforAgrobiotechnologyandBiologicalSchool,KeyLaboratoryforAgro-MicrobialResourceandA
• 出 处: 中国科学：生命科学英文版
• 发表日期: 2006年2期
• 关键词: PAENIBACILLUS massiliensis T7 nifBHDKENX nifB-lacZ inverse-PCR. Paenibacillus
• 基金项目: This work was supported by the '973' Project (Grant No. 001CB108904).

A 324 bp of nifH fragment was PCR amplified from Paenibacillus massiliensis T7 using the universal degenerate primers. The PCR-amplified nifH fragment was labeled with DIG and then used as a probe in Southern blot analysis. Southern blot result showed tha