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试验采用旋转回归设计方法，从１９８６～１９９４年在代表松嫩平原黑土区的东北农业大学试验地内进行了以农肥、化肥、秸秆、绿肥等培肥物质为试验因素的长期定位试验。试验分两个阶段进行，前一阶段为培肥阶段，到１９９２年结束。后一阶段为耗竭阶段，主要观测各培肥处理对土壤养分的持续效应及对产量的影响。建立了耗竭阶段农肥、化肥、秸秆对土壤有机质、全氮、全磷及产量的数学模型。随着耗竭试验的继续，各处理土壤有机质呈明显下降趋势。在农肥、秸秆、化肥三因素，以农肥对土壤有机质的贡献最大，秸秆次之，化肥最差。从每两个培肥物质的交互作用对土壤有机质的影响看，化肥和农肥、秸秆之间无明显的正交互作用。而秸秆和农肥间有较明显的正交互作用，说明了在农业生产中二者可互相代替，同样可以达到培肥的目的。
The experiment uses the method of rotation regression design. From 1986 to 1994, a long-term experiment was carried out in Northeast Agricultural University on the northeastern agricultural university on the Songnen Plain black soil to test the fertilizers including fertilizer, fertilizer, straw and green manure. The experiment was conducted in two stages, the previous stage being the stage of fertilization and ending in 1992. The latter stage is the depletion stage, the main observation of the fertilization treatment of soil nutrients continued effect and impact on yield. The mathematic model of soil organic matter, total nitrogen, total phosphorus and yield in the depletion stage was established. With the continuation of the depletion test, the soil organic matter of all treatments showed a significant downward trend. In the three aspects of agricultural fertilizer, straw and chemical fertilizer, the contribution of agricultural fertilizer to soil organic matter is the largest, followed by straw and chemical fertilizer is the worst. From the interaction of every two fertilizers on soil organic matter, there is no obvious orthogonal interaction between fertilizers and fertilizer and straw. The straw and the fertilizer between the more obvious interaction between the two, indicating that the two can replace each other in agricultural production, the same can achieve the purpose of fertilizer.